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ralb ha  (Addgene inc)


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    Addgene inc ralb ha
    Ralb Ha, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ralb+ha/pmc06679750-117-1-33?v=Addgene+inc
    Average 90 stars, based on 2 article reviews
    ralb ha - by Bioz Stars, 2026-07
    90/100 stars

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    ralb ha  (Addgene inc)
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    Ralb Ha, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ralb+ha/pmc06679750-117-1-33?v=Addgene+inc
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    pla cmv ha ralb  (Addgene inc)
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    Addgene inc pla cmv ha ralb
    Growth factors activate TBK1 in a TBKBP1-dependent manner. a,b , Immunoblot analysis of S172-phosphorylated (p-) and total TBK1 in the lysates of HEK293T cells transfected with TBK1 along with Kras G12D ( a ) or <t>RalB</t> ( b ). c,d , Immunoblot analysis of TBK1 S172 phosphorylation in EGF-induced A549 cells stably transduced with vector control or Kras G12D ( c ) and RalB ( d ). e , Immunoblot analysis of S172-phosphorylated (p-) and total TBK1 in whole-cell lysates of A549 cells stimulated by EGF, Insulin or FBS. f,g, Immunoblot analysis of S172-phosphorylated (p-) and total TBK1 in whole-cell lysates of EGF-stimulated lung cells from WT or Tank KO mice ( f ) or EGF-stimulated A549 cells stably transduced with a control shRNA (shCtrl) or shRNAs for Tank ( g , upper panel) and Nap1 ( g , lower panel). h,i , Immunoblot analysis of S172-phosphorylated TBK1 (p-TBK1) and the indicated other proteins in whole-cell lysates of control or Tbkbp1 -knockdown A549 cells stimulated with the indicated growth factors ( h ) or PRR ligands ( i ). Data are representative of three independent experiments. Unprocessed blots are shown in .
    Pla Cmv Ha Ralb, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ralb+ha/pmc06901116-212-14-23?v=Addgene+inc
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    Growth factors activate TBK1 in a TBKBP1-dependent manner. a,b , Immunoblot analysis of S172-phosphorylated (p-) and total TBK1 in the lysates of HEK293T cells transfected with TBK1 along with Kras G12D ( a ) or RalB ( b ). c,d , Immunoblot analysis of TBK1 S172 phosphorylation in EGF-induced A549 cells stably transduced with vector control or Kras G12D ( c ) and RalB ( d ). e , Immunoblot analysis of S172-phosphorylated (p-) and total TBK1 in whole-cell lysates of A549 cells stimulated by EGF, Insulin or FBS. f,g, Immunoblot analysis of S172-phosphorylated (p-) and total TBK1 in whole-cell lysates of EGF-stimulated lung cells from WT or Tank KO mice ( f ) or EGF-stimulated A549 cells stably transduced with a control shRNA (shCtrl) or shRNAs for Tank ( g , upper panel) and Nap1 ( g , lower panel). h,i , Immunoblot analysis of S172-phosphorylated TBK1 (p-TBK1) and the indicated other proteins in whole-cell lysates of control or Tbkbp1 -knockdown A549 cells stimulated with the indicated growth factors ( h ) or PRR ligands ( i ). Data are representative of three independent experiments. Unprocessed blots are shown in .

    Journal: Nature cell biology

    Article Title: TBKBP1 and TBK1 form a growth factor signaling axis mediating immunosuppression and tumorigenesis

    doi: 10.1038/s41556-019-0429-8

    Figure Lengend Snippet: Growth factors activate TBK1 in a TBKBP1-dependent manner. a,b , Immunoblot analysis of S172-phosphorylated (p-) and total TBK1 in the lysates of HEK293T cells transfected with TBK1 along with Kras G12D ( a ) or RalB ( b ). c,d , Immunoblot analysis of TBK1 S172 phosphorylation in EGF-induced A549 cells stably transduced with vector control or Kras G12D ( c ) and RalB ( d ). e , Immunoblot analysis of S172-phosphorylated (p-) and total TBK1 in whole-cell lysates of A549 cells stimulated by EGF, Insulin or FBS. f,g, Immunoblot analysis of S172-phosphorylated (p-) and total TBK1 in whole-cell lysates of EGF-stimulated lung cells from WT or Tank KO mice ( f ) or EGF-stimulated A549 cells stably transduced with a control shRNA (shCtrl) or shRNAs for Tank ( g , upper panel) and Nap1 ( g , lower panel). h,i , Immunoblot analysis of S172-phosphorylated TBK1 (p-TBK1) and the indicated other proteins in whole-cell lysates of control or Tbkbp1 -knockdown A549 cells stimulated with the indicated growth factors ( h ) or PRR ligands ( i ). Data are representative of three independent experiments. Unprocessed blots are shown in .

    Article Snippet: Flag-tagged TBK1 were provided by Dr. Chen Wang (Shanghai Institutes for Biological Sciences). pBabe-HA-Kras-G12D, pLA CMV-HA RALB, pRK5-HA-Raptor and pRK-5-Myc-Rictor were purchased from Addgene.

    Techniques: Western Blot, Transfection, Phospho-proteomics, Stable Transfection, Transduction, Plasmid Preparation, Control, shRNA, Knockdown